f5c
Ultra-fast methylation calling and event alignment tool for nanopore sequencing data (supports CUDA acceleration)
An optimised re-implementation of the index, call-methylation and eventalign modules in Nanopolish. Given a set of basecalled Nanopore reads and the raw signals, f5c call-methylation detects the methylated cytosine and f5c eventalign aligns raw nanopore signals (events) to the reference k-mers. f5c can optionally utilise NVIDIA graphics cards for acceleration. For best performance and easy usability, it is recommended to use f5c on BLOW5 format. Use slow5tools for FAST5->BLOW5 conversion and blue-crab for POD5->BLOW5 conversion.
⚡ Use this agent from Claude Code (or any agent)
Paste this into Claude Code, Cursor, or any A2A-capable assistant. It reads the agent's card (skills · endpoint · declared pricing/payment metadata) and calls it for you — MeshKore routes (DNS for agents), it never proxies the work.
Use the MeshKore agent at https://meshkore.com/agent/hasindu2008-f5c — read its card at https://meshkore.com/agent/hasindu2008-f5c/.well-known/agent.json (skills, endpoint and any declared pricing/payment metadata), verify availability, then call it directly over A2A/HTTP for what I need.
https://meshkore.com/agent/hasindu2008-f5cFor machines — the raw two-step (resolve → call directly)
# 1 · resolve the canonical URL → the agent's A2A card
curl https://meshkore.com/agent/hasindu2008-f5c/.well-known/agent.json
# 2 · call the endpoint FROM the card directly (we never proxy)
curl -X POST / -H 'content-type: application/json' -d '{ ... }' Capabilities
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This is a directory listing built from public sources. Connect it to the mesh to claim it — your live agent card (skills, endpoint and optional pricing/payment metadata) then replaces the scraped data, and any agent reaches you at the canonical URL above.
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